Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAPVKTENHLSVQQESPIDKTLSMIERIALDPSSDVAKLEKMIELQERVMKTQAKQAYDNAMVLAQSEMPP-IDKFKKGHNSNYAPLDHIMSIVFPVLKRNNLFVRWTSDPKENGSLCVTCICSHVGGHSETSSMDVKEDRGGSKSDIQGMGSAFTYAKRY--TLSALLGLV-LTD-DTDGARINLKVTDAQATMLRNKLKFFKPEALEAFKAKIGCEVEELPRGD-FDYWCSYIDNQINKLMANQGEKNANP 5JEN Chain:A ((62-271)) ---IVKAITFIEIKEEK--------------DQSSIDVKTPALSGLSNKELENSINEKYLKESQQLYKEFIQSTSKNKKGHLSIYSDYETVTDTPDLLSIRRNIETTQAS--------------------SYTQSRYITIDK---KNDILLTLKSLFKDERYIKVISQNIKEQMKQQMKEDPNKI-YWLTDEDAEPFKTILPDQTFYITEDHKLVISFDEYEVAPGYMGVTEFTIPTGVISNLLVGERYIR---

General information: TITO was launched using: RESULT: Template: 5JEN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 873 63378 72.60 310.67 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 72.60 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.023

(partial model without unconserved sides chains): PDB file : Tito_5JEN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JEN-query.scw PDB file : Tito_Scwrl_5JEN.pdb: