Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MKVLRVLVTTTALLAAGAAMADEAVVHDSYAFDKNQLIPVGARAEVGTTGYGGALLWQANPYVGLALGYNGGDISWTDDVSVNGTKYDLDMDNNNVYLNAEIRPWGASTNPWAQGLYIAAGAAYLDNDYDLAKRIGNGDTLSIDGKNYQQAVPGQEGGVRGKMSYKNDIAPYLGFGFAPKISKNWGVFGEVGAYYTGNPKVELTQYNLASVTGNPTSAQDAVDKEANEIRNDNKYEWMPVGKVGVNFYW---------------------------------------------------------------------------------------------------------------------- 5DXF Chain:B ((18-528)) PKEFEGKGKLKLSGRILNVMTSLPLQIVRGNSALYSSQHFLAENKEWETHLIAWTGDEEDKSKIEKKLCDASGTPNIHPVWLLRRDQGRWRKYAENVLWPVFHYIQPSDGKAETDAWHDYVKFNEAYLNKIKSVYKPGDIIWIHDYYLLLLPQLLRMEFPNAYIGFFLHVPFPSSEYFRCLSKRSQLLDGMLG-ADKIGFQ-SDSFQRH-FISCCARVLGCEVN--------------------------RDSVSAYG-----------TTISVETLPIGIDTEKIEHDAF--SSELGVEEKVQALKQVYKGKKLIV-GRDRLDKVRG-------------VIQKLEGFEIFLDMYPEW------------RETVVLIQVSSPGYSHS-----ANVETRVTEIISRINSKYGNLNHTPVLHYQMRVAKEEYLALLRVADLALITSVRDGMNTTSLEFVICQKYNNSPLILSEFTGTATVLKDAIMVNPWDSVGVAKTINDALMLSTKEKVSLESKLYEKVLSNTVQNWTSTFICDILSHSI

General information: TITO was launched using: RESULT: Template: 5DXF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 668 17602 26.35 100.01 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : 26.35 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.008

(partial model without unconserved sides chains): PDB file : Tito_5DXF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DXF-query.scw PDB file : Tito_Scwrl_5DXF.pdb: