Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINIQNTILGKIVDRKHEDLAARLKQRNLQDVEELAKAATPVRGFANALQ----HKRPGVIAEIKKASPSKGIIRADFNPAEIAQQYEQAGAACLSVLTDVDFFQGADENIAIARNHCTLPALRKDFLVDPYNVVEARALHADCILLIVACLSDQQLEEMSKTAFEHQLDVLVEVHDEEELERALKLSEQCLLGVNNRNLKTFDVDLNTTIRLKKLLPASRLLITESGIATPDDVRMMQEHDIHSFLVGESFMKQPRPDQAFTALFGQPQTV 3TSM Chain:A ((8-268)) ------DILRKIEAYKREEIAAAKARLALDELKARTRDQSAPRGFLKALEAKRAAGQFALIAEIKKASPSKGLIRPDFDPPALAKAYEEGGAACLSVLTDTPSFQGAPEFLTAARQACSLPALRKDFLFDPYQVYEARSWGADCILIIMASVDDDLAKELEDTAFALGMDALIEVHDEAEMERALKLS-SRLLGVNNRNLRSFEVNLAVSERLAKMAPSDRLLVGESGIFTHEDCLRLEKSGIGTFLIGESLMRQHDVAAATRALLTG----

General information: TITO was launched using: RESULT: Template: 3TSM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1295 18863 14.57 73.40 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : 14.57 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_3TSM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TSM-query.scw PDB file : Tito_Scwrl_3TSM.pdb: