Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQVYLVGGAVRDYLLGHSYQEKDYVVVGATPEHMLAQGFQPVGKDFPVFLHPETKEEYALARTERKSGQGYHGFQFFTD---TTV-SLEDDLIRRDLTINAIAMDQDGKIYDPYGGQNDLENKILRHVSEA---FAEDPLRVLRVARFAARYFPYGFQIAPETLQLMQTMADSGELDALTPERVWKETSRALMENHADIYFQTLRDCGALKHLFPEIDALFGVPQRPEYHPEVDCGIHTLMSLQQACKSNYSLDVRFAVLVHDLGKALTPAKELPRHIMHEERGIKPVTQLCERLRVPTQTKQLALSVCKEHLKCHQIMSLKPGTLWRLLQRLDVLRRPERVEAFVQACECDAKGRLGLEDRPYPQAQYMREAMQIVRSIKVQDLPENIKGAEIGEMLIQYRIEALAEFKNQHQSLSHS 1MIY Chain:A ((23-180)) ---YFVGGAVRDLLLGRPIGDVD-IATSALPEDVMAI--------FPKTIDVGSKHGTVVVV---HKGKAYEVTTFKTDGSVTFVRSLEEDLKRRDFTMNAIAMDEYGTIIDPFGGREAIRRRIIRTVGEAEKRFREDALRMMRAVRFVSE---LGFALAPDTEQAI------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1MIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 639 -22309 -34.91 -157.11 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -34.91 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_1MIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MIY-query.scw PDB file : Tito_Scwrl_1MIY.pdb: