Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDETKMSYQSIFRPDAFANKVIIVTGGGSGIGRCTAHELAALAAQVVITGRKIEKLEKVSQEII-EDGGLVHFIVCDNREEEQVKNMIAEVIEKFGKLDGLVNNAGGQFPSALENISANGFDAVVRNNLHATFYLMREAYNQWMAKHGGSIVNMTADMWG-GMPGMGHSGAARSGVDNLTKTASVEWGKSGVRVNAVAPGWIV-SSGMDNYSGDFAKVIIPSLAGNVPLKRMGTESEVSSAICYLLSDAAAFVSGVTLRIDGAASQGTRMYPLAEATNSQSYNGFHRAFIPEIFKKDKTEKVEQSNNSGSE 4FC6 Chain:D ((10-270)) -DDCLPAYRHLFCPDLLRDKVAFITGGGSGIGFRIAEIFMRHGCHTVIASRSLPRVLTAARKLAGATGRRCLPLSMDVRAPPAVMAAVDQALKEFGRIDILINCAAGNFLCPAGALSFNAFKTVMDIDTSGTFNVSRVLYEKFFRDHGGVIVNITATLGNRGQALQVHAGSAKAAVDAMTRHLAVEWGPQNIRVNSLAPGPISGTEGLRRLGGPQASLSTKVTAS--PLQRLGNKTEIAHSVLYLASPLASYVTGAVLVADGGA-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FC6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1465 -104356 -71.23 -404.48 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.76 3D Compatibility (PKB) : -71.23 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_4FC6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FC6-query.scw PDB file : Tito_Scwrl_4FC6.pdb: