Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKTIILGGFTEMKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAALITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDG-FQYFQSS-NESDYIPNIDQAL-NAGFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVAAAYTT--------ETNVVGFIGGVKGEVIDRF-DAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDKGRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYTLNGEKKNFTLTSTLKAVGT-VVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTE------GQLSDEAKKAVDEAKEKIISGDVKVPETPEEN 4IIL Chain:A ((31-344)) ---------------------------------------------------RAVRIAVFV-PGFRHDSPVYAMLCDGVERAVTQERAT-GRSIGLDIIEAGPNQALWREKLAHLAAEQRYRLIVSSNPALPHVLEPILRQFPLQRFLVLDAYAPQEHSLITFRYNQWEQAYLAGHLSALVSASAMRFANADKKIGLIAGQSYPVMTQTIIPAFLAGARAV-D---PAFEVDVRVVGNWYDAAKSADLARILFHEGVDVMMPICGGANQGVLAAAREL------GFYVSWFDDNGY--------ARAPGYVVGSSVMEQERLAYEQTLRC-IRGELPSAGAWTLGVKDGYVRFIEEDPLYLQTVPEPIRVRQSALLRRIQSGELTLP------

General information: TITO was launched using: RESULT: Template: 4IIL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1754 20528 11.70 69.58 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 11.70 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_4IIL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IIL-query.scw PDB file : Tito_Scwrl_4IIL.pdb: