Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MMKKEIVLTAA-----------MMLFSMVASTTFVSATEVYPKEYNTEG------------TITFEAGDEGVTPPVDPE-NPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAAADVMNDGSRKP-TYVQVTD-RRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVSLVD----------PTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWV--------YRFGANENENQDAVQLSVPGKSVKLAQQYSTKLVWTLEDTPNN---------------------------------- 4LDV Chain:A ((16-356)) SDALCRELWHACAGPLVTLPREGERVYYFPEGHMEQLEASMHQGLEQQMPSFNLPSKILCKVINIQRRAETDEVYAQITLLPELDQSEPTSP-DAPVQETVHSFCKTLTASDTSTHGGFSVLRRHADDCLPPLDMSQQPPWQELVATDLHNSEWHFRHIFRGQPRRHLLTTGWSVFVSSKKLVAGDAFIFLRGENEELRVGVRRHMPSSVISSHSMHIGVLATAAHAITTGTIFSVFYKPRTSRSEFIVSVNRYLEAKTQKLSVGMRFKMRFKRFSGTIVGVQENKSSVWHDSEWRSLKVQWDEPSSVFRPERVSPWELEPLN

General information: TITO was launched using: RESULT: Template: 4LDV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1040 15813 15.20 65.34 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 15.20 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_4LDV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LDV-query.scw PDB file : Tito_Scwrl_4LDV.pdb: