Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MIKGAIFLRPKQQLFITLLVILGALFNNISV-SASAFNFSVTPITS----ENQIDKRKTYFDLQLDP------DQEVEVKAELRNDTEKEVKIDIS-------------------VNSATTNSNVMVEYGKN---EIEKDMS--------------LIFDLVDYVSYPQIVTLKPKSVQTVAFH---------VRMPNERFD-----GVL--AGGITFQEIQTEKDQTETKDQ-SLSIENEYT-YIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQN----DQ--KTYINQVVIETKITKKGHSE-----------------------VLYQEEKEGLQIAPNTNFS---FPTALNGQPLTPGEYHLTMTVLGNENENG--------------KFSRKKGN-----TTINYTNQWVFE---KDFTIDGKVAKELNTTDVTIKKDNNWIYILVASLFLL------------FILLCILLFVLRKKEEKEK 4C3X Chain:A ((23-530)) DWTSECDVLVVGSGGGALTGAYTAAAQG--LTTIVLEKTDRFGGTSAYSGASIWLPGTQVQERAGLPDSTENARTYLRALLGDAESERQDAYVETAPAVVALLEQNPNIEFEFRAFPDYYKAEGRMDTGRSINPLDLDPADIGDLAGKVRPELDQDRTGQDHAPGPMIGGRALIGRLLAAVQSTGKAELRTESVLTSLIVEDGRVVGAEVESGGETQRIKANRGVLMAAGGIEGNAEMREQAGTPGKAIWSMGPFGANTGDAISAGIAVGGATALLDQAWFCPGVEQPDGSAAFMVGVRGGLVVDSAGERYLNESLPYDQFGRAMDAHDDNGSAVPSFMIFDSREGGGLPAICIPNTAPAKHLEAGTWVGADTLEELAAKTGLPADALRSTVEKFNDAAKLGVDEEFHRGEDPYDAFFCPPNGGANAALTAIENGPFYAARIVLSDLGTKGGLV--TDVNGRVLRADGSAIDGLYAAGNTSASLSGRFYPGPGVPLGTAMVFSYRAAQDMAK

General information: TITO was launched using: RESULT: Template: 4C3X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1908 36364 19.06 100.73 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 19.06 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.037

(partial model without unconserved sides chains): PDB file : Tito_4C3X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C3X-query.scw PDB file : Tito_Scwrl_4C3X.pdb: