Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------MNHQAAGGQLVGNIYFERM--------FIMFHVFYEFQFVEIVKEF------FELPYKSDNSLSF----DSAFQNCAKPEWNKIKNKKRNFTSVAKQEFDFFRITNSPEYVPANNEIFSEVGYIKLLNKFMK--YAITFDDV---TIKKFFQY------------------EEFSRDLISELVNFMMTQSKPYKL----TNDAQFVLEKIE---LITDDNGIFKLGQIGWKSQKRLIPLLKKEK-ETLSPINIASILFELWSTKKIAPCPLDPTKIKEKEKKKEKVEKQEKDNKHSNLYIEVNLDYPEDVP---FPNGNYYTLYIKN------KRNSLYYGEWDGLKHVAKEKKGIFKKVK--GDLYQEIEKIRKNQEISQERNLYIVV---NKENISIIHSNIEFWS---------------------------SFENKVRSVKSKLSTLAHQNKIIGYEASTLEEQTIFNDFLPSWKWQLLSPEIDLDRHLFELETE----------- 5FJ8 Chain:O ((31-654)) VMTISSLEQRTLNPDLFLYKELVKAHLGERAASVIGMLVALGRLSVRELVEKIDGMDVDSVKTTLVSLTQLRCVKYLQETAISGKKTTYYYYNEEGIHILLYSGLIIDEIITQMRVNDEEEHKQLVAEIVQNVISLGSLTVEDYLSSVTSDSMKYTISSLFVQLCEMGYLIQISKLHYTPIEDLWQFLYEKHYKNIPRNSPLSDLKKRSQAKMNAKTDFAKIINKPNELSQILTVDPKTSLRIVKPTVSLTINLDRFMKGRRS----KQLINLAKTRVGSVTAQVYKIALRLTEQKSPKIRDPLTQTGLLQDLEEAKSFQDEAELVEEKTPGLTFNAIDLARHLPSASLINSHLKILASSNFPFLNETKPGVYYVPYSKLMPVLKSSVYEYVIASTLGPSAMRLSRCIRDNKLVSEKIINSTALMKEKDIRSTLASLIRYNSVEIQEVPRTADRSASRAVFLFRCKETHSYNFMRQNLEWNMANLLFKKEKLKQENSTLLKKANRDDLLPS-ELNQLKMVNERELNVFARLSRLLSLWEVFQMA

General information: TITO was launched using: RESULT: Template: 5FJ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 1344 89165 66.34 220.70 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain O : 0.62 3D Compatibility (PKB) : 66.34 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_5FJ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FJ8-query.scw PDB file : Tito_Scwrl_5FJ8.pdb: