Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLGANQKHKNYYEGPKVIVTWALGHLLGLKMPEDLNK-EWQ--SWQMDTLPMIP-KKLGIK--PLPKTGHQLKAIKQLAQRKDVSEAVIATDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESALARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITLQAGK-----------EQAKMLQK--------------------NQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEIQREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFAPEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENR---ARY-----EKLSNEERKIYQMIVSRFLGLFAQPHKVSQTKVTVEF----GKERFIFRQNRVVQAGWKGETETE-----------T--ETVKWEKGMRINP-DFAIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPKGKQLLVLVNP---SLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQEYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRKMEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAAALKAAMKK 5D5H Chain:A ((37-619)) ----------YLGS-GYIVESSRGHIRDLPRAASDVPAKYKSQPWA--RLGVNVDADFEPLYIISPEKRSTVSELRGLLKDV--DELYLATDGDREGEAIAWHLLETLKPRIPVKRMVFHEITEPAIRAAAEHPRDLDI--DLVDAQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVARERDRMAFRSAAYWDILAKLDASVSDPDAAPPTFSARLTAVAGRRVATGRDFDSLGTLRKGDEVIVLDEGSATALAAGLDGTQLTVASAEEKPYARRPYPPFMTSTLQQEASRKLRFSAERTMSIAQRLYEN-GYITYMRTDSTTLSESAINAARTQARQL--YGD---EYVAPAPRQYTRKVKNA--QEAHEAIRPAGETFATPDAVRRELDGPNIDDFRLYELIWQRTVASQMADARGMTLSLRITGMSGHQEVVFSATGRTLTFPGFLKAYVETVDELVGGEADDAERRLPHLTPGQRLDIVELTPDGHATNPPARYTEASLVKALEELGIGRPSTYSSIIKTIQDRGYVHKKGSALVPSWVAFAVTGLLEQHFGRLVDYDFTAAMEDELDEIAAGNERRTNWLNNFYFGGDHGVPD----------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5D5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2513 151429 60.26 292.90 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 60.26 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_5D5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D5H-query.scw PDB file : Tito_Scwrl_5D5H.pdb: