Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------MGYDKHQARTSGWRVPENNLIFVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILI--YFFH------------------------------------------------------------------------------------------------------------------------------- 5JUF Chain:A ((1-299)) MNLKDSIGLRIKTERERQQMSREVLCLDGAELTVRQLIRIEKGESLPSLDRLSYIAKRLGKSMTELLDQDNITIPDEYYEMKNRLIKFPTYRNPDRIKSKLTLIEEVYEKFFDILPEEELLTLDILENILSFTSWEESPKVEEIYEDLFEQVKRKRKFS-------TNDLLVIDYYFFHLYGRKQYDKKLFERIIKRVLNQEIWTDDVYNIVLFNDLMAIAALKIFHNSFSDFLTVVDKALAVIEKSQLYSYKPSVFVLKAKYELLHKENKKEAAENYDKAIVFASVLEDSVLEESIKAGKLADGL

General information: TITO was launched using: RESULT: Template: 5JUF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 191 726 3.80 12.09 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 3.80 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.108

(partial model without unconserved sides chains): PDB file : Tito_5JUF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JUF-query.scw PDB file : Tito_Scwrl_5JUF.pdb: