Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRDIRDLKIVKAPDENGVSRFVLFREKGTP-ETRTEEEDRLVQMIEDIVLKVERVHFLTIVNTLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF 3LAJ Chain:F ((18-153)) --RAGRQARIVAILSSAQVRSQNELAALLAAEGIEVTQATLSRDLEELGAVKLRGADGGTGIYVVPEDGSPVRGVSGGTDRMARLLGELLVSTDDSGNLAVLRTPPGAAHYLASAIDRAALPQVVGTIAGDDTILVVA----------------------

General information: TITO was launched using: RESULT: Template: 3LAJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 452 -59253 -131.09 -438.91 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain F : 0.78 3D Compatibility (PKB) : -131.09 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_3LAJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LAJ-query.scw PDB file : Tito_Scwrl_3LAJ.pdb: