Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPERIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASRGGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNANGWDKPDIWMAKTIIPRPNSQ 1N71 Chain:D ((2-179)) -IISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPERIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASRGGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNANGWDKPDIWMAKTIIPRP---

General information: TITO was launched using: RESULT: Template: 1N71.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 811 -94148 -116.09 -528.92 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.99 3D Compatibility (PKB) : -116.09 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.772

(partial model without unconserved sides chains): PDB file : Tito_1N71.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N71-query.scw PDB file : Tito_Scwrl_1N71.pdb: