Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGKSTYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSIYENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDV--KDKLHKSALSLSGGQQQRVCIARVLAVNPEIILLDEPTSALDPVSTGKIESMLLEL-RERYTMIIVTHNMSQASRISDKTAFFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG 2OLK Chain:D ((23-263)) ---QMIDVHQLKKSFGSLEVLKGINVHIREGEVVVVIGPSGSGKSTFLRCLNLLEDFDEGEIIIDGINLKAKD-----TNLNKVREEVGMVFQRFNLFP---HMTVLNNITLAPMKVRKWPREKAEA--KAMELLDKVGLKDKAHAYPDSLSGGQAQRVAIARALAMEPKIMLFDEPTSALDPEMVGEVLSVMKQLANEGMTMVVVTHEMGFAREVGDRVLFMDGGYIIEEGKPEDLFDRPQHERTKAFLSKVF-

General information: TITO was launched using: RESULT: Template: 2OLK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1257 -31185 -24.81 -131.03 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : -24.81 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_2OLK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OLK-query.scw PDB file : Tito_Scwrl_2OLK.pdb: