Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGINIENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPDMPVTLVGDNGVTKATTVRALLPYAFTEKDL 1UX1 Chain:C ((3-130)) ----RQELITEALKARDMAYAPYSKFQVGAALLTKDGKVYRGCNIENAAYSMCNHAEQTALFKAVSEGDTEFQMLAVAADTPGPVSPCGACRQVISELCTKDVIVVLTNLQGQIKEMTVEELLPGAFSSEDL

General information: TITO was launched using: RESULT: Template: 1UX1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 643 -80077 -124.54 -625.60 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain C : 0.86 3D Compatibility (PKB) : -124.54 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_1UX1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UX1-query.scw PDB file : Tito_Scwrl_1UX1.pdb: