Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEG-RLAGYIVDAEAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMH-THRILNIITQPEGIPQGVMIRAIEPAAMIDQMSKNRGGKTG-PDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKKKTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGSINIFR 1BNK Chain:? ((1-216)) KGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISS--QGDGACVLLRALEPLEGLETMRQLRSRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERA------VVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQD------------------

General information: TITO was launched using: RESULT: Template: 1BNK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1156 -9424 -8.15 -47.84 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -8.15 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_1BNK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BNK-query.scw PDB file : Tito_Scwrl_1BNK.pdb: