Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPRADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDH-LDVMGPLGNGFDLSNLHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRL-GETRIATDDGTYGMHGNVGNLLLGTK---TEPTAVFACGSNGLLKTVEQLFSSH-QNVQLSLESRMACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII 5JFC Chain:S ((6-256)) ---MMFKILRKERLAPGINLFEIESPRIAKHAKPGQFVMIRLHE--KGERIPLTIADVDISKGSITIVAQEVGKTTRELGTYEAGDYILDVLGPLGKPSHIDY---FGTVVMIGGGVGVAEIYPVAKAMKEKGNYVISILGFRTKDLVFWEDKLRSVSDEVIVTTNDGSYGMKGFTTHALQKLIEEGRKIDLVHAVGPAIMMKAVAELTKPYGIKTVASLNPIMVDGTGMCGACRVTV-GG----EVKFACVDGPEFDAHLVDW

General information: TITO was launched using: RESULT: Template: 5JFC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain S - contact count / total energy / energy per contact / energy per residue : 1443 -72316 -50.11 -295.17 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain S : 0.73 3D Compatibility (PKB) : -50.11 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_5JFC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JFC-query.scw PDB file : Tito_Scwrl_5JFC.pdb: