Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQERLSVINELVADFEKENPDIKIKQVPVEE-------SAFNTKIVTLARSGKLPEVMEVS-Q-DFAKVMDKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKLSEAGFS---EPENWDDILKIAQHFTDKENK---QYGIAMPTAEGTMSEQAFSQFALSNGANVLDDEGEVTI---DTEKMREALSFYQNLSQY-TM-P-G------SNDVTEIKDAFMNGTAPMAIYSTYILPSVYEAGNSENIGFA-IPTQKDQ-A--VYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKWVLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKMGDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK 4R9F Chain:A ((41-441)) -----------------------------KQVVLTLWYPWAG-PDGDAVVSLAKEYSKTHPNVQIKAQMVSGAGIAATQGGGQGKFLSAVAAGNPPDLVLYWGQDALPGLADQGAIIPLDDYLK---DVDTSKFFEAAYNAMK-YKGKIY-GLPEMVNVRVLFWNKDLFKQAGLDPNTPPKTIAELDQMAAKLTKTKNGTIEQMGFIPWIGQ-----GVPHVMAGVFGTSLVDSNGNPILSPDKNPQLLNLLKWEVSYSDKYGAMNINKFIAGMSQNSSQANDPFVLGKVAMMISGEWQINANKQYNPKLNFGVGPIPQAPGGKPMPSLMDGNTWMIPKG--SKHPQEAMDFIKWTMDPQRIADTADK-VYNIAPIVEAAKI-QKLNNDPYFKEVLN-------VAQKGSI---YYTPAAKGMLST--ETAANNAFQAAQYKKSTPEQALKNAQAEA-

General information: TITO was launched using: RESULT: Template: 4R9F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2092 105557 50.46 285.29 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 50.46 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_4R9F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R9F-query.scw PDB file : Tito_Scwrl_4R9F.pdb: