Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAISIKHPGSVDVLE-QIEVPVPKPKQGELLIKVHTAAVNRTDIMKRE-SVSLKPPYPIL-GVEVAGEVVENKSDISTFTPGTKVYGLVNL-GGYAEYAVMPADRAILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQLSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGASYWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLG-TLLTPRSDQYKAELAQTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHE-YMEANKNIGKIILKITE 1YB5 Chain:B ((30-349)) MRAVRVFEFGGPEVLKLRSDIAVPIPKDHQVLIKVHACGVNPVETYIRSGTYSRKPLLPYTPGSDVAGVIEAVGDNASAFKKGDRVFTSSTISGGYAEYALAADHTVYKLPEKLDFKQGAAIGIPYFTAYRALIHSACVKAGESVLVHGASGGVGLAACQIARAYG-LKILGTAGTEEGQKIVLQNGAHEVFNHREVNYIDKIKKYVGEKGIDIIIEMLANVNLSKDLSLLSHGGRVIVVGSR-GTI--EINPRDTMAKESSIIGVTLFSSTKEEFQQYAAAL---QAG--MEIGWLKPVIGSQYPLEKVAEAHENIIHGSGATGKMIL----

General information: TITO was launched using: RESULT: Template: 1YB5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1880 -25124 -13.36 -80.01 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -13.36 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_1YB5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YB5-query.scw PDB file : Tito_Scwrl_1YB5.pdb: