Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDTSKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARFAAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAY-VPQNRERIFLIGHSRDACTEQV-FPIIGSSPTS-DQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGDEGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQEVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQGFPDWAFDRASQVN-SDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL 1SVU Chain:B ((12-318)) LRFIDLFAGLGGFRLALESCGAECVYSNEWDKYAQEVYEMNFGEKPE---GDITQVNEKTIPDH---DILCAGFPCQA-------------RGTLFFDIARIVREKKPKVVFMENVKNFASHDNGNTLEVVKNTMNELDYSFHAKVLNALDYGIPQKRERIYMICFRNDLNIQNFQFP----KPFELNTFVKDLLLPDSEVEHL---VIDRKDLVMT---NQEIEQTTPK----TVRLGIVGKGGWGERIYSTRGIAITLSAYGGG----------------------------------IFAKTGGYLVNG----------KTRKLHPRECARVMGYPD-----SYKVHPSTSQAYKQFGNSVVINVLQYIAYNI--------

General information: TITO was launched using: RESULT: Template: 1SVU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1650 -100908 -61.16 -347.96 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -61.16 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_1SVU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SVU-query.scw PDB file : Tito_Scwrl_1SVU.pdb: