Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEHRSYYAIIPANVRYD---KRLKPNTKLLYGEITALC---------NERGFCWA--GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKRNTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF 4PT7 Chain:C ((13-100)) ----KERFYQIPKVFFTSENYKNLTNDMKIAYAILRDRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PT7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 177 -2877 -16.25 -38.88 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : -16.25 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_4PT7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PT7-query.scw PDB file : Tito_Scwrl_4PT7.pdb: