Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFTTYTTFPEQTSNESLWILVDSEQLQ--------SN----LNTYQINNLESILTATQFKANFNETLPLFGQLSTQPHSQLLGLGKAAELQAAKLAKLAQTIIKSAQN-KFKHIAIDIAAL-------PVEYHYLFALSLTQAAYGYDEFKSTKNEFVLQQVDLISSQT-SLDENQLALVHAVQSGQSYARDLGNRPGNICFPEYLAEQALALAAEFPDLLKVTVLNEQQMADLGMYAFLAVSKGS-ERPGRIVTLEYQAQ-L-EQAPVVLVGKGVTFDTGGISLKPGL--GMDEMKFDMCGAASVLGTIRALCEARL-PIHVVGAIAAAENMPSGKATRPGDIVTTMSGQTVEILNTDAEGRLVLCDTLTYIKRFNPAVVIDIATLTGACVVALGKVLSGLFSPDDTLAAELQQAGEQSFDRVWRMPVIDDYQELLDSPFADIANIGGPHGGAITAACFLERFTR-DYRWAHLDVAGTAWLSGSAKGATGRPVPLLMQFLANRVSTNG 4KSI Chain:A ((32-529)) --IGAKDTAVVQWQGDLLAIGATENDMARDENSKFKNPLLQQLDSELNGLLSAASSEEDFSGKSGQSVNLRF---PGGRITLVGLGSSASS-PTSYHSLGQAAAAAAKSSQARNIAVALASTDGLSAESKINSASAIATGVVLGSFEDNRFRSESKKSTLESLDILGLGTGPEIERKIKYAEHVCAGVILGRELVNAPANIVTPAVLAEEAKKIASTYSDVISVNILDAEQCKELKMGAYLAVAAAATENPPYFIHLCFKTPTKERKTKLALVGKGLTFDSGGYNLKVGARSRIELMKNDMGGAAAVLGAAKALGEIRPSRVEVHFIVAACENMISAEGMRPGDIVTASNGKTIEVNNTDAEGRLTLADALIYACNQGVEKIIDLATLTGAIMVALGPSVAGAFTPNDDLAREVVEAAEASGEKLWRMPMEESYWESMKSGVADMINTGPGNGGAITGALFLKQFVDEKVQWLHLDVAGPVWSD-EKKNATGYGVSTLVEWVLRN-----

General information: TITO was launched using: RESULT: Template: 4KSI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2725 3902 1.43 8.30 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 1.43 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_4KSI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KSI-query.scw PDB file : Tito_Scwrl_4KSI.pdb: