Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYHGPVSRNAAE-HLLSSG-INGSFLVRESESSPGQRSISLR-----YEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHH 1BHF Chain:A ((9-91)) WFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHY

General information: TITO was launched using: RESULT: Template: 1BHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 259 2150 8.30 28.28 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 8.30 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_1BHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BHF-query.scw PDB file : Tito_Scwrl_1BHF.pdb: