Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYHGPVSRNAAE-HLLSSG-INGSFLVRESESSPGQRSISLR-----YEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHH 1LKL Chain:A ((5-87)) WFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHY

General information: TITO was launched using: RESULT: Template: 1LKL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 255 3840 15.06 50.53 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 15.06 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_1LKL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LKL-query.scw PDB file : Tito_Scwrl_1LKL.pdb: