Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLRSAMHASDVDDAGRALQSTQRSIVSISRLTNEMSEVRAMSEGSFGVVKCYRHDFDKLEYAVKQTKRPICG---ESNLQQQLQEIYALSSFPHRHIVRYFDGWVEDRAVFVRLEKLDDC-M-ASLP------PPVSESVLTAMLHQTSTALYELHSHDVVHMDVKPENILRRQLDADTFIFKLCDFGLARPLNGKDSVTGEHFLGLNDDDGDRRYMSPELLKNLHDVVGPPADMYALGKSCEAMMTA--TEDPSNTS-------------ARHLESYSPAFIALIESMLCEDPARRPSAFQVVQATLPERLLSDGGLLELQRRIDAIRCEISDLDASDNDVMSSPRT 3C0G Chain:B ((19-292)) ----------------------------DVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHRDVKPHCVLLASKE-NSAPVKLGGFGVAIQLGESGL-------VAGGRVGTPHFMAPEVVKRE--PYGKPVDVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKE-------------------------------------

General information: TITO was launched using: RESULT: Template: 3C0G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1120 -18786 -16.77 -75.75 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -16.77 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_3C0G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C0G-query.scw PDB file : Tito_Scwrl_3C0G.pdb: