Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDSKQCEAFLAVAEVGSFDAAGEYLCITPSAVSLRVQALEKYLGQILIIRGR-PCVLTQAGQTLLQHLRHTRLMEQNLLQGLMGKSSDSEFYKIALASNADSLATWLLPSIQQTLFKE-KIVLELKIDDQSHTHTLLETGQVNACITAEEQ---VMAGCLAQPLGKMRYKMLASAEFVNRWFSGGINREVLRKTPAVIFNHKDLMHSEVLLKGYGL-PMQSYPYSFIPATDAFVKAIQLGLGYGMVPELQVQPLLENETLVDIM--PE-AQLDVPLYWHHWKRQSKQLDVLTETIVESAKQILR 2ESN Chain:B ((7-305)) RLDLNLLLVFDALYRHRNVGTAASELAISASAFSHALGRLRQGLDDELFLRQGNRMQPTQRAEHLAAAVAAALRALGEGLEEWRPFVPGQSQRTFVFAATDYTAFALLPPLMNRLQHSAPGVRLRLVNAERKLSVEALASGRIDFALGYDEEHERLPEGIQAHDWFADRYVVVARRDHPRLA--GAPTLEGYLAERHAVVTPWNEDS-GVIDRLLARSGLRREVAVQLPTVLAALFLAGSTDFLLTAPRHAARALAEAAGLALYPAPFDIPPYVLRLYSHVQ----DAHAWMIGQLKGLDIS---

General information: TITO was launched using: RESULT: Template: 2ESN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1290 -67393 -52.24 -235.64 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -52.24 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.328

(partial model without unconserved sides chains): PDB file : Tito_2ESN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ESN-query.scw PDB file : Tito_Scwrl_2ESN.pdb: