Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQSSAALNSPNPMKKKVITALIAVAIGHIGVLWAVSHIKPAELKPIDKKPLQVRFVKIQEQPKPLPPKPKE-----PPKKPEPKKEVKEVKVVE----KPVTPPKKVEKIQQVKKAETP----------KETVKTEPKVETKVVTTT---TTVTEKVVEKPKPVVQEAPKAQPA-----PDPSPKRVSIGGSG-----VQWSRSPR--------LTVSPKDLQGEARSVVVLIEAD--------EKGKIINVRVTRSSGISSLDDKVVRAVRGAKFKPYMENGVAYPIRAEQPFDLNP---------------------------------------------- 4FHL Chain:A ((2-392)) GSMTLSSNQYQLPLNVRPYTTTWCSQSPSCSNLLAIGHDTGITIYCASEESTGLTLQELFTIQTGLPTLHLSFSSSCSYSENSPVYSLFLACVCQDNTVRLIITKNETIITQHVLGGKSGHHNFVNDIDIADVYSADNRLAEQVIASVGDDCTLIIWRLTDEGPILAGYPLSSPGISVQFRPSNPNQLIVGERNGNIRIFDWTLNLTELVKNPWLLTLNTLPLVNSSLANVRWIGSDGSGILAMCKSGAWLRWNLFKNLLPNVQGISLFPSLLGACPHPRYMDYFATAHSQHGLIQLINTYEKDSNSIPIQLGMPIVDFCWHQDGSHLAIATEGSVLLTRLMGFT

General information: TITO was launched using: RESULT: Template: 4FHL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1337 31703 23.71 126.30 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 23.71 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.124

(partial model without unconserved sides chains): PDB file : Tito_4FHL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FHL-query.scw PDB file : Tito_Scwrl_4FHL.pdb: