Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIMQIRQFQAQRMQAPRDFTPIENTPICVEIGAGKGKHALLFSGQNPQHTLYAIERTREKFLAMQKQHGLEPRDNLIPVHADALPWVVHALYPAQVEHFFILYPNPEPHNPAQRWLNMPFFEFLLSRLKTGGTITLAGNIPEYIE-EAEQQLLNVWKLPYQKEKIAQTSARTHFEIKYLER-GELCQQLIMTKPEGYNTRFDEFAPLQGQIHVE 3HM2 Chain:B ((6-178)) GQLTKQHVRALAISALA----PKPHETLWDIGGGSGSIAIEWLRSTPQTTAVCFEISEERRERILSNAINLGVSDRIAVQQGAPRAFDD--VPDNPDVIFIGLTAPG------------VFAAAWKRLPVGGRLVANAVTVESEQMLWA-------LRKQFGGTISSFAISHEHTVGSFITMKPALPVHQWTVVKA------------------

General information: TITO was launched using: RESULT: Template: 3HM2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 813 -72462 -89.13 -428.77 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -89.13 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_3HM2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HM2-query.scw PDB file : Tito_Scwrl_3HM2.pdb: