Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSQEPNQSLFNQASTSIQACIECMFACKHCASACLQEEHVHMMRKCIKHCLTCVETCQLCTSLELRHSELAEQAMQLCANACQLCAAECSKHEHEHCQVCAKACLACAQACQAYRA 4ZSZ Chain:B ((192-295)) ------YNFLEEEIYERCRLAAHHCIHACERYLALCTESS-----REQRQHAGDCADLCRLAALLLERRSPWAPAACELAARYALACAERCDGD-EPLERECAGACRRFVAACHPL--

General information: TITO was launched using: RESULT: Template: 4ZSZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 456 -76691 -168.18 -737.41 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -168.18 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.643

(partial model without unconserved sides chains): PDB file : Tito_4ZSZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZSZ-query.scw PDB file : Tito_Scwrl_4ZSZ.pdb: