Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIKEKTLDDLLNSTYTFIL------ENGDHITA-SKGENFEVRNAVLTLENPRSRLSISE-TRSKLISCIGEFFWYLNGSDSLEFIEYYISDYRKYIEYQIDEN-KPALGAYGPRIFGA----QNQFNKIIEVLKDKPTSRRAVISIYSKDDLLREDSRDIPCTCLLQFFIRNERLHLTATMRSNDAAIGLVHDIFSFTLIQELLLAKLIRYIPNLQLGEYTHIVGSLHIYKKI 5B6E Chain:A ((2-223)) ----------FGTFQDAYLSQLRDIYHSPEFRNAPRGQASRERIGAGFRLLDPVQRHISVPARRANVVFNFAEALWYLSGSDRLDFIQYYAPGIAAYSA----DGRTLRGTAYGPRIFRHPAGGVNQWENVVKTLTDDPDSKRAVIQIFDPRELAVADNIDVACTLALQFLIRDGLLCGIGYMRANDAFRGAVSDVFSFTFLQEFTAR-----YLGLGIGTYHHVVGSVHIYDSD

General information: TITO was launched using: RESULT: Template: 5B6E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 882 -17882 -20.27 -88.09 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -20.27 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_5B6E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5B6E-query.scw PDB file : Tito_Scwrl_5B6E.pdb: