Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQHQSLFNKSQWTQLVKHFSAVAVLGLTIAAPAWAIDPTAKELRIGFQKSSINFAIAKQQKLFEQEFPNAKITWNEFPAGPQILEALAVGSLDVGVTGDTPPVYAQAAGKPLYYIAYEAAKP-LASAILVPKNSQLKQLKDIKGKRIALQKGSSSHY-LLVQAVRKAGLKWSDITPIWL-TPADARAAFQKGAVDAWAIWDPYYAS-AQLEDQARVLV---SGKGLSPN------YTFYLAAPNFVKQYPKAVPGLIKQINQADKWVQSYQVETASAI--GQSTGLKPATSDLFIKRR-PRPSSAAPLNSKVIAEQQQIADIFTQQGIIPKPISIKQAV---WGAK------- 3QSL Chain:A ((28-345)) ----QKLEKAKVQIAVGGKPLIYYLPLTIAEV---------------------------KGFFKDE--GLDVSIADFAGGSKALQAVVGGSADVVSGAFEHTLSLQAKGQFYRAFALQGRAPMIGVGVSKKNLPGYKGPADLKGRKIGVTAPGSSTNMVVNFFLAKHGLKASDVSFIGVGAGAGAVTALRSGQIDAISNTDPV-VSMLETSGDIQIIVDTRTLKDTKEIFGGNMPAGCLYAPQAFVDANPNTAQALTNAIVRADKWIQKAGADEIAKAVPEGYLLGDPAVYKAAIGKSMEGLSPDGVIPEDGAATALKALAAF-VPDFDAAKVDPAKAWTNEYTRRANEKYPN

General information: TITO was launched using: RESULT: Template: 3QSL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1849 19210 10.39 65.79 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 10.39 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_3QSL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QSL-query.scw PDB file : Tito_Scwrl_3QSL.pdb: