Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYGEVMNTIQKIELDTAKLLINGQFLESKTQEWQDIVNPATQEVIGCVPFATVEEVDAAIQAAQDAFASWRQTPIQARMRIMLKLQDLIRANMKEIAQVLTAEQGKTLADAEGDIQRGLEVVEHACSVGTLQMGEYVEGVARGVDTYTLQQPLGVCAGITPFNFPAMIPLWMFPMAIVCGNTFVLKPSEQDPLSTMMLVELAIQAGIPAGVLNVVHGGKEVVDRLCTHKDIKAISFVGSTAVGTHVYNLAGQHGKRVQAMMGAKNHVVVMPDANKEQTLNALVGAAFGAAGQRCMALSVAVMVGDS--KQWIQELVEKAKILKVNAGHEPNTDIGPVISKRAKARVIDLINSGVEQGAELLLDGRNVQVQGYESGNFVGATIFSGVNTDMRIYKEEIFGPVLSIICVDTLDEAIALINANPFGNGVGLFTQSGAIARTFQNLIDIGQVGINIPIPVPVPFFSFTGSRGSKLGDLGPYGKQAVQFYTQTKTITSRWFEDSHEVGGVNTTISLR 4ZZ7 Chain:E ((4-484)) --------------MTTIGHLINGQLVTENTRS-QNVFNPATGEIGKQLDLASTKTVEQAISAAQHAFPTWRNTPPLKRARVMFRFKELLEQHADEICRLIGEEHGKIAHDAMGELQRGIENVEYACGAPELLKGEHSRNVGPGIDSWSEFQPMGVVAGITPFNFPVMVPLWMFPMAIVCGNCFVLKPSERDPSSTLYIAQLLQEAGLPDGVMNVVNGDKEAVDALLHDDRVKAVSFVGSTPIAEYIYRTASANGKRCQALGGAKNHAIVMPDADMDNAVNQLLGAAFGSSGERCMALSVAVAVGDAAGDALVSKMTQAMQKLKVGPSTDSGNDFGPVITRQHQEKVIGYINSAEQQGATIVVDGRQPKVPNHENGFFVGGTLIDHVTPEMTSYQEEIFGPVLQVVRVATMQDAMDLIDAHEYGNGTCIFTRDGEAARYFSDNIQVGMVGINIPLPVPVAYHSFGGWKRSLFGDLHAYGPDAVRFYTKRKTVTQRW-----------------

General information: TITO was launched using: RESULT: Template: 4ZZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 3030 -155526 -51.33 -324.69 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain E : 0.84 3D Compatibility (PKB) : -51.33 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_4ZZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZZ7-query.scw PDB file : Tito_Scwrl_4ZZ7.pdb: