Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKQVINISGYHMNIPNVLKYYFTETFLKTAIRKPSQLNLPPTALRPMLEQLCRAFPKQKNVTVRPIRLAGIKGEEIKAQDSATQLIF-HIHGGAFFLGSLKTHHAFMTDLAARTQMQIIHVDYPLAPEHPYPEATEALYDVYQSLLVQGIQPKDIILSGDSCGANLALALCLRLKEQPELMPSGLILLSPFLDLTLTSESLRFNQKHDALLSLEALQIGIQHYIGNHIAADDPRVSPIFDNLEGLPPTLVQVGSKEILLDDAKRFREKAEEAGVKVHFKLYTGMWHNFQMFNAWFEEAKQAMADIAEFAHELDKT 4XVC Chain:E ((49-311)) ---------------------------------------------RRLYETMLGSMPLDDDIQTERLGVNGVPAEWIYAPGARDDQVFLYLHGGGYVIGSMRTHRVMLSHIARAAGCRVLGLDYRLAPETPFPAPVEDTVAAYRWLLAHGYDPSRIALGGDSAGGGLVVAALVALRYIGEPLPAAGVCLSPWIDMEATGESFTTNATMDPSVNKERVMSIAALYLGGK-NPQAPLASPLYADLQGLPPLLVQVGGIETLLDDARALTTRAKAAGVDADLEVWDDMPHVWQHFAPILPEGKQAIARIGEF-------

General information: TITO was launched using: RESULT: Template: 4XVC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1627 -67215 -41.31 -256.55 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain E : 0.75 3D Compatibility (PKB) : -41.31 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.589

(partial model without unconserved sides chains): PDB file : Tito_4XVC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XVC-query.scw PDB file : Tito_Scwrl_4XVC.pdb: