Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLMVAVFAGMVPLASFSAQAATPEQECQKLQDDYNLIYASKGFCFKDQDAKEKYGNENCHTTKPKF------SDKEQQRLDAIK-------ERQKELKCK 1IOW Chain:? ((1-96)) MTDKIAVLLGGTSAEREVSLNSGAAVLAGLRE-GGIDAYPVDP-----KEVDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMD

General information: TITO was launched using: RESULT: Template: 1IOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 365 -6594 -18.07 -79.45 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -18.07 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_1IOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IOW-query.scw PDB file : Tito_Scwrl_1IOW.pdb: