Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFNCDLVWMNSMSALLRFTQFVQK-----TFALWVIIFAALALWQ-----PEFFVWLKAYIPWILGIIMLGMGMTMTVDDFKGVLQSPKAVLIGVVAQFVVMPGLAFILCKLFNLPPEIAVGVILVGCC---------------------------------PGGTASNVITYM----------AKGNVALSVACTSVSTLLAPVLTPAIFYLLASQWLKIDAASMFISILQVV--LLPI-------------VIGLILRTWLKR-------QVESYIQVMPLVSVIAIVAIVAAIIGGSKAA--ILQSGLLILAVVILHNGLGYLLGFAAARFFKLPYADSKAIAVE--VGMQNSGLGVALAAVHFAA------------SPITAVPSAIFSLWHNIS-GPALATYWASKHKQE---- 4CZB Chain:A ((1-415)) MELMMAIGYLGLALVLGSLVAKIAEKLKIPDIPLLLLLGLIIGPFLQIIPSDSAMEIFEYAGPIGLIFILLGGAFTMRISLLKRVIKT--VVRLDTITFLITLLISGFIFNMVLNLPYTSPVGYLFGAITAATDPATLIPVFSRVRTNPEVAITLEAESIFNDPLGIVSTSVILGLFGLFSSSNPLIDLITLAGGAIVVGLLLAKIYEKIIIHCDFHEYVAPLVLGGAMLLLYVGDDLLPSICGYGFSGYMAVAIMGLYLGDALFRADDIDYKYIVSFCDDLSLLARVFIFVFLGACIKLSMLENYFIPGLLVALGSIFLARPLGVFLGLIGSKH---SFKEKLYFALEGPRGVVPAALAVTVGIEILKNADKIPASITKYITPTDIAGTIIIGTFMTILLSVILEASWAGMLALKLLGE

General information: TITO was launched using: RESULT: Template: 4CZB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1345 -171983 -127.87 -539.13 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -127.87 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_4CZB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZB-query.scw PDB file : Tito_Scwrl_4CZB.pdb: