Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRAISHIDTFQGLILALQNYWAEQGCVVLQPYDMEM---------GAGTFHTA-TFLRALGPETWNAAYVQPSRRP----KDG-RYGENPNRLQHYYQFQVVLK----------------------P--NPDNIQQLYLD----------SLK-AIGIDTLTHDI--------RFVEDN-WESPTLGAWGLGWEVWLN-------------------GMEVTQFTYFQQV----GG-VECYPVTGEITYGLERLAMYLQGVDSVYDLVWTKGQFGTVTYGDVFHQNEVEQSTYNFEYAPVDKLFELFDFYESEANRLMEAKLPLPAYEQVVKASHTFNLLDARGAISVTERQRYILRVRTLARAIAQSYVQARAELGFPMAEPHLRDEVLAQLKAQAESEAAKAEKN 3HY0 Chain:A ((2-257)) --------KSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYT----ARHHTFFEMLGNFSFGDYFKLDAILFAWLLLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPR-ERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTAMGLERIAAVLQHVNSNYDID-------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3HY0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 650 2743 4.22 15.85 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 4.22 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_3HY0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HY0-query.scw PDB file : Tito_Scwrl_3HY0.pdb: