Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSDRIREKLQILADAAKYDVSCSSSGSDRKNKNKGLGDASHSGICHSYTEDGRCVSLLKIL-FSNVCIFDCAYCVSRRSNDVQRAAFTVQEVVDLTINFYRRNYIEGLFLSSGIFKSADHTMERMLQVVKKLRLEENFNGYIHLKTIPGASPELIHEAGLYADRMSINLEMPTEIGLKTFAPEKSHQEVQKDLGLVRDRLIQLKDERQIIKHVPKYVPAGQTTQMVVGAHQESDQDVLFMADKHYKEFKLKRVYFSGYIPINNENNYLPAVGSAPPLLRENRLYQSDWLMRFYGFEVNEIVNEKHPNLDLDVDPKLSWALRHPEQFPVDLNRADYQMILRVPGIGVKSAKKIVQARRFGKIHIDLLKKLGVAYQRAKFFIRCEDSPKFQKELSSSFIRQQILTQGSSKYVQQLSPQLSLGF 3T7V Chain:A ((12-348)) SLGDKVIEGYQL--TDNDLRTLLSLESKEGLERLYSAARKVRDHYF------GNRVFLNCFIYFSTYCKNQCSFCYYNCRNEINRYRLTMEEIKETCKTLKGAGF-HMVDLTMGEDPYYYEDPNRFVELVQIVKEELGLPIMISPGLMDNATLLKAREKG--ANFLALYQETYDTELYRKLRVGQSFDGRVNARRFAKQQGYCVEDGILT--------GVGND-------IESTILSLRGMSTND--------PDMVRVMT------FLPQEGTPLEGFRDKSNLSELKIISVLRLMFP------------------------KRLIPASLDLEGIDGMVLRLNAGANIVTSILPPDSQ----------------LEGVANY--DRDLEERDRDIKSVVRRLEIMGMKPARQADFEAVL---

General information: TITO was launched using: RESULT: Template: 3T7V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1905 22516 11.82 67.21 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 11.82 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_3T7V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T7V-query.scw PDB file : Tito_Scwrl_3T7V.pdb: