Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIYNAPLADMKFILNDVFKAEQFWQANEKLAHVDAATAEAILEEMAKFAQNVTHPLNRTGDEEGARYENGEVFTPAGFKDAFRQYAEGGWIGLGADEEWGGQGMPKMLTVLADEMLFATNPSFMLYPLLSVGAGMALSSYASQEQKETYLPKIYSGEWSGTMCLTEPHAGTDLGIIKTKAERNEDG-TYNITGTKIFITGGDNDLAENIIHLVLAKTPDAPAGSRGISLFIVPKYLVNEDGSLGDRNHVGPGSIEHKMGIKASATCVMNFDGAKG---YLVGKENEGLAAMFVMMNYERLSMGIQGLGASEFAYQNAAQYATARLQGRSAAGVQSPSKPADSILVHGDVRRMLLNVRANNEASRAFAVYVGQQLDITKFSTD-AEAVKKANDRVALLTPIAKAYLTDTAFQATLDAQMVFGGHGYIREWGMEQCIRDLRIAQIYEGTNGVQSQDLIGRKTIK-CGGAFIAEYITEIRDFANDLDTDLNFIKDATLDAATEI-EAITQFIIE-QAAENVEFPNSAAVDYLHAVGLLSFSYMFAKIAAAAKDKSGDFYQ-NKLALAQYFVERILPDIDARIAKIKAGSDLIMGFSEDYFTNQA 2Z1Q Chain:B ((5-577)) ---KKLWQKGGGWLLEVPERVYTPEDFDESVKEIARTTRTFVEREVLPLLERMEHG------------------ELELNVPLMRKAGELGLLAIDVPEEYGGLDLPKVISTVVAEELSGSGGFSVTYGAHTSIGTLPLVYFGTEEQKRKYLPKLASGEWIAAYCLTEPGSGSDALAAKTRATLSEDGKHYILNGVKQWISNAGF----AHLFTVFAKV-----DGEHFTAFLVER----------DTPGLSFGPEEKKMGIKASSTRQVILEDVKVPVENVLGEIGKGHKIAFNVLNVGRYKLGAGAVGGAKRALELSAQYATQRVQFGR------------PIGRFGLIQQKLGEMASRIYAAESAVYRTVGLIDEALLGKKGPEAVMAGIEEYAVEASIIKVLGSEVLDYVVDEGVQIHGGYGYSQEYPIERAYRDARINRIFEGTNEINRLLIPGMLLRRAEPEDLELHQVQNLKKLALMVAGLAVQKYGQGVEEEQEVLGAVADILIDAYAAESALLRARRLGGLAPVLARIYLAQALDRAQAGALSVLPRLVEGDEARVVYSAARRLTKREPGDLVALRRQAAEAVLEAGGYPIPR-

General information: TITO was launched using: RESULT: Template: 2Z1Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3084 -86192 -27.95 -159.61 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -27.95 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_2Z1Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z1Q-query.scw PDB file : Tito_Scwrl_2Z1Q.pdb: