Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPISNQDLRKAGLKVTLPRIKILELLENSKQHHLSAEDIYKTLLEQGEDVGLATVYRVLTQFEAAGIIQRHHFENNHSVFEIMQEDHHDHLVCQNCNKVIEFTNDVIEKEQHSVAEQHGFTLTGHSLNLYGYCNEPECQEALRKK 5FD5 Chain:D ((15-133)) -----------GMRMTEQRRVIARILEDSEDHP-DVEELYRRSVKVDAKISISTVYRTVKLFEDAGIIARHDFRDGRSRYETVPEEHHDHLIDLKTGTVIEFRSPEIEALQERIAREHGFRLVDHRLELYG--------------

General information: TITO was launched using: RESULT: Template: 5FD5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 343 -42860 -124.95 -360.16 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -124.95 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_5FD5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FD5-query.scw PDB file : Tito_Scwrl_5FD5.pdb: