Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENNLILPALILVPFIAGFTCWLVDKLDKTLPRWIALIGMLITLGLGLALWQSGTYSYELGSKVPTWTAEFYLPWIQ--SLGIGIHLAVDGLSLLMVLLTALLGVLAVGCS--WGEIQKNVGFFHLNLLWSLGGVIGVFLAIDLFLFFFFWEMMLVPIYFLIALWGHKGSNGRSRVYAATKFFLYTQIAGLVMLIGILGLVVYGYMMTGMIGFDYNYLLAVANRLPAGFAYGFMICFFIGFAVKLPVFPLHGWLPDAHAQAPTAGSVDL-AGILIKTAAYGLLRFVIPFFPAASAQFADIAIIFGLIGIFYGAWCAYQQTDMKRLLAYTSISHMGFVLLAIYAGNILTFQGLMIMMLAHGLSSAALFIMSGQIYERL-HTRDLRLMGGLRGQLQYLPFFLMFFVAALIGVPGLGNFIGEFLILMGSFNKY--PVFTIIASISLVFAGLYGLILIHRALFGEPNSEQKQHYTSPLKDLSAREVSILMICAIGLVWLGIYPQTFLDVSHSSMQWLVNSYVPVQEFVETVQQTATQLDHVEIQ 5LNK Chain:L ((65-468)) -----------------------------------------------------------------------NWHWLTIQTLKLSLSFKMDFFSMMFVPVALFVTWSIMEFSMWYMHSDPNINQFFKYLLLFLITMLILVTANNLFQLFIGWEGVGIMSFLLIGWWYGR----TDANTAALQAILYNRIGDIGFILAMAWFLINLNT------WDLQQIFMLN----PNDSNLPLMGLILAATGKSAQFGLHPWLPSAM-EGPTPVSALLHSSTMVVAGIFLLIRFYPLT-EN-NKFGQSIMLCLGAMTTLFTAMCALTQNDIKKIIAFSTSSQLGLMMVTIGINQP---HLAFLHICTHAFFKAMLFMCSGSIIHSLNDEQDIRKMGGLFKAMPFTTTALIIGSLALTGMPFLTGFYSKDLIIESANTSYTNAWALLMTLVATSFTAIYSTRIIFFALLGQPRFPTL--INI--NENNPFLINSIKRLLIGSLFAGFII-----------------------------------------

General information: TITO was launched using: RESULT: Template: 5LNK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 2132 -313645 -147.11 -792.03 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain L : 0.67 3D Compatibility (PKB) : -147.11 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_5LNK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LNK-query.scw PDB file : Tito_Scwrl_5LNK.pdb: