Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALS-NRPEIVEVAPFSAETNAALDAI-VSNFSEEDANRIKEIERTTNHDVKAVEYFLKEKIAGIAELQNAGEFIHFACTSEDINNLSHALMLKNGREVL-VSSMKQILNAISALATTHAEQPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPNVDWPAHSQAFV-ESLGLTFNPYTTQIEPHDYMAELFDALRRFNTILIDFNRDVWGYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENSEGNLGIANAVLAHLGEKLPISRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKGIGKLELNANRLNEDLDQAQEVLAEPIQTVMRRYNVEKPYEKLKALTRGQAMTRDMMVDFVNGNELAQVPSEERARLAELTPATYTGNAAEQAKQINELISKI 4EFC Chain:B ((19-464)) -NPLFALSPLDGRYKRQTKALRAFFSEYGFFRYRVLVEVEYFTALCKDVPTIVPLRSVTDEQLQKLRKITLDCFSVSSAEEIKRLERVTNHDIKAVEYFIKERMDTCG-LSHVTEFVHFGLTSQDINNTAIPMMIRDAIVTLYLPALDGIIGSLTS-KLVDWDVPMLARTHGQPASPTNLAKEFVVWIERLREQRRQLCEVPTTGKFGGATGNFNAHLVAYPSVNWRAFADMFLAKYLGLKRQQATTQIENYDHLAALCDACARLHVILIDMCRDVWQYISMGFFKQKVKEGEVGSSTMPHKVNPIDFENAEGNLALSNALLNFFASKLPISRLQRDLTDSTVLRNLGVPIGHACVAFASISQGLEKLMISRETISRELSSNWAVVAEGIQTVLRRECYPKPYETLKK------VTEEQVRNFING--LTDISDDVRAELLAITPFTYVG----------------

General information: TITO was launched using: RESULT: Template: 4EFC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1935 -45485 -23.51 -104.56 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -23.51 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_4EFC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EFC-query.scw PDB file : Tito_Scwrl_4EFC.pdb: