Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------MGLNMPEPTSTTAATTTSLAA---VSLLPFINGNA---LLGAVLGAAFVAYLEKDLSAKQRIVFMLLCV------------GFGYLLGPEITSRTQYISSDATASMVAAIFAI-----YILI---KLLDWVKNSTLAQLWKTFRGGGNS--- 2J9L Chain:A ((9-182)) HKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFPVVVSRESQRLVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPLPPYTPPTLKLRNILDLSPFTVTDLTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANFNEFLEV

General information: TITO was launched using: RESULT: Template: 2J9L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 436 -27296 -62.61 -221.92 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -62.61 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_2J9L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J9L-query.scw PDB file : Tito_Scwrl_2J9L.pdb: