Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTKLLQAIYRDSDLSEKTASMIEQKV-SVYQQKKG--------QAGKLHWDEE--DIFLITYGDQFFEEQQPTLRTFKKFYQK--YLADT-FRVVHFLPFFPYT---SDDGFSVVNYQAINDRLGTWEDIEEMRSET-----RLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT---D----TSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLEKGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKDNISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLASHDGIGLNPVR------GILPEKTILELVEQLKK-EGARVNYKKNPDGTTSPYEINATYLDA--------LSSPTDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEK---TGENRKINRQKFLLADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSAD-SFISFIREAKEEKVLVIHNFSNQSAGYLLPKG-----DYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK 1ZS2 Chain:A ((44-627)) --MNELDSVYGNNEALLPMLEMLLAQAWQSYSQRNSSLKDIDIARENNPDWILSNKQVGGVCYVDLFA-------GDLKGLKDKIPYFQELGLTYLYLMPLFKCPEGKSDGGYAVSSYRDVNPALGTIGDLREVIAALHEAGISAVVDFIFNHTSNEHEWAQRCAAGDPLFDNFYYIFPDRRMPDQYDRTLREIF-PDQHPGGFSQL-EDG--RWVWTTFNSFQWDLNYSNPWVFRAMAGEMLFLANLGVDILRMDAVAFIWKQMGTSCENLPQAHALIRAFNAVMRIAAPAVFFKSQAIVHPDQVVQYIGQ--DECQIGYNPLQMALLWNTLATREVNLLHQALTYRHNLPEHTAWVNYVRSHDDIGWTFADEDAAYLGISGYDHRQFLNRFFVNRFDG--SF-----ARGVPFQYNPSTGDCRVSGTAAALVGLAQDDPHAVDRIKLLYSIALSTGGLPLIYLGDEVGTLNDDDWSQDSNKSDDSRWAHRPRYNEALYAQRNDPSTAAGQIYQGLRHMIAVRQSNPRFDG-GRLVTFNTNNKHIIGYIRN---NALLAFGNFSEYPQTVTAHTLQAMPFKAHDLIGGKTVS--LNQDLTLQPYQVMWLEI--

General information: TITO was launched using: RESULT: Template: 1ZS2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3078 -16232 -5.27 -30.57 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -5.27 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_1ZS2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZS2-query.scw PDB file : Tito_Scwrl_1ZS2.pdb: