Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVEPPGASVKP---TEAARRR--VFKIDGEEFTP--LETDCLIRIFKNKKTVHALFVP----PGTELDWRLDHNHYAVGIKHNCDIIIGWKPSSWRIEGDKVSSLADGFSVVTTKSTD----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 3DJL Chain:A ((3-540)) WQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLASIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHNLAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPLLSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGLEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPFVAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQNDLLLRATGGVC

General information: TITO was launched using: RESULT: Template: 3DJL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 221 2088 9.45 19.51 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : 9.45 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.225

(partial model without unconserved sides chains): PDB file : Tito_3DJL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DJL-query.scw PDB file : Tito_Scwrl_3DJL.pdb: