Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------CTYSVYDSIG-KMVVEDKDLPTNRRDYYTIGKKKYTFYFKENCDFDYSKPPLPRCWHVSGIPKGLYDQVQGMEQPCPAQ----------------------------------- 1ECS Chain:A ((2-121)) TDQATPNLPSRDFDSTAAFYERLGFGIVFRD-------AGWMILQRGDLMLEFFAHPGLDPLASWFSCCLRLDDL-AEFYRQCKSVGIQETSSGYPRIHAPELQGWGGTMAALVDPDGTLLRLIQNEL

General information: TITO was launched using: RESULT: Template: 1ECS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 208 -5223 -25.11 -74.61 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -25.11 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_1ECS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ECS-query.scw PDB file : Tito_Scwrl_1ECS.pdb: