Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GPCVITIDGPYNPKYNFYPSHGRGVTGPHGQVVIGDFTCTVGATC-----NQLTCNAPQPEYSILGVDSSKWGLSDTESDSDSDSNSKQAIMPHNRKQVAPVSHY 1HKY Chain:A ((1-86)) -------DYKDDDDKVKLTCYQNGVSFTGGK-AISEAKAASSQACQELCEKDAKCRFFTLASGKCSLFADDAALRPTKSDG-AVSGNKRCILLED----------

General information: TITO was launched using: RESULT: Template: 1HKY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 322 8008 24.87 98.86 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 24.87 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_1HKY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HKY-query.scw PDB file : Tito_Scwrl_1HKY.pdb: