Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------STSTKVKLENRKEPAL--CHVVIIKPAQIRHPLDTWVETFAWVKAGKVV-----------EWHSPKYGSVKVKTDEDDCHLTFLVNELINGYTCQLSR-------------- 1TTW Chain:A ((3-120)) TYSSLLEEFATELGLEEIETNELGHGAVTIDKIWVVHLAPINEKELVAFMRAGILTGQSQLYDILRKNLFSPLSGVIRCALDKDD-HWLLWSQLNINDTSGTQLASVLTSLVDKAVTLS

General information: TITO was launched using: RESULT: Template: 1TTW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 177 -8043 -45.44 -95.75 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -45.44 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.203

(partial model without unconserved sides chains): PDB file : Tito_1TTW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TTW-query.scw PDB file : Tito_Scwrl_1TTW.pdb: