Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------ACSIELLNVNQA----VVDTKCIPFDTTTAMKDPTGPGGGQLYKVHVNNLCGIGLDDGQELANGASLRKLSSP--- 4RS8 Chain:A ((9-92)) YIFLTPRAYIIVHLLKVGKAKASEISENTQIPYQTVIQNIRWLLAEGYVVKEQKGEEIYYKLTDKGKQMATAELEKIRKLVEVV

General information: TITO was launched using: RESULT: Template: 4RS8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 241 -991 -4.11 -14.36 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -4.11 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.082

(partial model without unconserved sides chains): PDB file : Tito_4RS8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RS8-query.scw PDB file : Tito_Scwrl_4RS8.pdb: