Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFHG-IPPWRSSRIIKQQGLVDGLFLLRDSQSNPKAFVLTLCHHQKIKNFQILPCEDDGQTFFSLDDGNTKFSDLIQLVDFY 5EEL Chain:D ((19-100)) WFHGRISREESQRLIGQQGLVDGLFLVRESQRNPQGFVLSLCHLQKVKHYLILPSEEEGRLYFSMDDGQTRFTDLLQLVEFH

General information: TITO was launched using: RESULT: Template: 5EEL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 281 -8916 -31.73 -110.07 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.92 3D Compatibility (PKB) : -31.73 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.791

(partial model without unconserved sides chains): PDB file : Tito_5EEL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EEL-query.scw PDB file : Tito_Scwrl_5EEL.pdb: