Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MIQWWQILL-LTLYSAYQICDEL----TIVSSAGSPVFAGFITGLIMGDVTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATA--FSVSQGIDAPLAITTIAV-----PVAALLTYFDVLGRMTTTFFA-HRVDAAIERFDYKGIERNYLLGAIPWALSRALP--VFFALAFGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLG--FAILLRYLPVKRNLHYLAMGFGLTAMLTVLYSYVTGLGGAVAGIVGTLPAEVAQKIGFVNNFKGLSMIGISIVGIFLAVLHFKNSQKVAVAAPSTPSESGEIEDDEF----------------------------- 5DBK Chain:A ((5-359)) DLRDVEEDVKGKLDEWLNALVHLDKQQVERIYEELQGEMKHVLDFEIINYYKLLYTRYLIMKRDISALEEELDKLKKVYKKYSPFQKLLYMYGRGLLCCLQYRWKDGLDYLLKTEVMAKEQGYHETGLYYNIALAYTHLDIHHLAIHFVNMALEGFRSEAKARNIINCQILIAVSYTEKGQYEEALKMYESILREATSFADKDVLLAITLSNMGSIYYKKGKYQQAKKYYLDSLQLQKQIDLNYLDTIYEMALVCIKLEELEEARTLIDKGIDAAKQEERFNAKLYLLLMLRYKYFEEAKDYKAFLENE----AIPLYKSAGNKIELKKVYVELAEHFSSLSRFEESNRYYRLVIDLMN

General information: TITO was launched using: RESULT: Template: 5DBK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1237 -30515 -24.67 -102.74 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -24.67 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_5DBK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DBK-query.scw PDB file : Tito_Scwrl_5DBK.pdb: